Coding

Part:BBa_K3861016:Design

Designed by: Kristin Funke   Group: iGEM21_Humboldt_Berlin   (2021-10-01)


AIDA autotransporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 773
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon optimized for Salmonella Typhimurium and forbidden restriction sites were removed by introduction of silent mutations without changing the amino acid sequence.


Source

References